Q-278. Antimicrobial Properties of a Recently Patented Formulation

N. Rocco1, R. Wheeler1, C. Esiobu2, N. Esiobu2, J. A. Rosenzweig1;
1Nova Southeastern Univ., Ft. Lauderdale, FL, 2Florida Atlantic Univ., Davie, FL.

The threat posed by bacteria to human health and quality of life is more unique today than ever. Multiple drug resistant strains of bacterial pathogens are quite common and lurk in a plethora of environments. To prevent transmission of these microbes, numerous hand gels, primarily alcohol based, have been designed to neutralize and kill transient flora acquired through fomites. Because of the need for a more rapid and sustained microbial killing of certain exposed surfaces, an antimicrobial formulation has been developed that relies on short chain surfactants in a potent matrix. We sought to evaluate the antimicrobial activity of this formulation on known surrogates of clinical significance (E. coli, S. aureus, Y. enterocolitica, M. smegmatis and the nosocomial threat P. aeruginosa). Bacteria or B. subtilis spores, at a concentration of ~104/mL, were suspended in either phosphate buffered saline (PBS) or Third Stream Bioscience (TSB) antimicrobial liquid at a 1X or 2X concentration. In vitro antimicrobial activity was evaluated by the Kirby Bauer disk diffusion assay and the microbroth dilution technique at 0, 2, 5, 10 minutes, 24 hours and 48 hours. All microbroth test bacteria were reduced by at least four logs after a 2 minute exposure to the formulation. Zones of inhibition ranged from 15 to 27mm revealing relative strain susceptibilities, and were higher at 30°C than at 37°C for E. coli, S. aureus and M. smegmatis. The mycobacteriocidal activity of this novel product will have significant public health benefits in the control of Mycobacterial diseases. Preliminary assays showed that the tested formulation is non-sporicidal. Subsequent studies will guide the formulation of sporicidal versions of the product and determine their mechanisms of action. Ultimately, the TSB antimicrobial was shown to be a highly effective antibacterial agent.