Q-270. Evaluation of Commercial Technologies for Bio-Decontamination of Personnel Protective Equipment-Relevant Surfaces

L. S. Smith, L. Wallace, S. S. Shah, V. K. Rastogi;
US Army, Aberdeen Proving Ground, MD.

Following the tragic events of 2001, dissemination of anthrax-causing spores - one of the deadliest bio-weapon agents - via letters sent through USPS led to a limited number of deaths and contamination of a large number of government and commercial building interiors. In the context of homeland security, these and other cases involving ricin toxin demonstrated an urgent need to develop countermeasures and clean up a variety of surfaces relevant to building interior and personnel protective equipment (PPE) and equipment used by the first responders. In this study, four surfaces - polycarbonate, steel, tyvek, and butyl rubber - highly relevant to the PPE used by first responders were intentionally contaminated with either spores (1E7) of plasmid-free avirulent ΔSterne Bacillus anthracis or ricin protein toxin (50 μg). Two disinfection technologies, pH-adjusted dilute (1/10th) Clorox bleach and dilute (1/6th) Peridox™ (Clean Earth Technologies, LLC) were tested for their ability to decontaminate with 15 minutes or 30 minutes contact time. Peridox is a commercially available disinfectant which is 24% hydrogen peroxide. The results from this study indicate that >6 log kill of spores was observed with 15 minute treatment of both the technologies. Ricin was decontaminated after a 15 minute treatment of dilute Clorox, while ricin was not decontaminated after a 30 minute treatment of Peridox. Based on this lab-scale study, the results will be discussed with respect to providing recommendation for decon standards, which should be targeted for PPE used by the first responders.