Q-268. Vaporized Hydrogen Peroxide (VHP) Decontamination of a New BSL-3 Laboratory

E. N. Ahanotu, T. Hewitt, A. Mosley, M. Henenin-Azer, T. Ravita;
SRA International Inc. (Constella Hlth. Sci.), Stone Mountain, GA.

BSL-3 laboratories (clinical and research laboratories) must be equipped with suitable room decontamination systems to complement the wipe-down techniques for surface and material disinfection. The objective of this study was to evaluate the efficacy, practicality and safety of vaporized hydrogen peroxide (VHP) decontamination technology in decontamination of a new BSL-3 laboratory. The facility was a new BSL-3 research laboratory that requires a pathogen-free environment. VHP fumigation was performed by a biodecontamination system which controlled all phases of the decontamination process. Decontamination efficacy was demonstrated using paper strip biological indicator (BI), strips containing spores of Bacillus atrophaeous prior to fumigation and culturing the BI after fumigation. The results indicated a complete inactivation of the Bacillus atrophaeous spores due to a lack of growth after incubation of the spore strips in trypticase soy broth (TSB) growth media. This suggested that the biodecontamination system was successful. This work demonstrates the utility of using VHP for the decontamination of a new BSL-3 laboratory which has various instrument and equipment surfaces. It also demonstrates that it is not necessary to partition the laboratory into individual smaller compartments prior to fumigation. VHP technology is a safe, practical and effective means of decontaminating a BSL-3 laboratory.