Q-265. The Correlation between Enteric Viruses and Male-Specific RNA Coliphages in the Well Water

S. F. Y. Yong1, J. J. Tee1, S. P. Lau1, Y. F. Ngeow2;
1Monash Univ., Bandar Sunway, MALAYSIA, 2Natl. Pub. Hlth. Lab. Sungai Buluh, Selangor, MALAYSIA.

Many studies have indicated males-specific RNA coliphages (FRNA) is the potential surrogate of enteric viruses in different types of water matrices. It assists in routine monitoring viral quality of water and food samples. A duration of 6-months water sampling from 3 chosen wells (GWA, GWB and GWC) was conducted to evaluate the potential of FRNA coliphage as a surrogate of enteric viruses. The GWA locates next to a paper manufacturing factory and is 5 km away from GWB on the higher land. The GWB locates in a poultry farm and is 10 km away from GWC. The GWC locates at down hill next to several furniture manufacturing factories. One hundred liter water was collected using a diaphragm pump from the 16 meter depth well and concentrated using a 5 inched cartridge electropositive charged alumina filter (Virocap filter). The viral particles were eluted and further concentrated using organic flocculation prior to plaque analysis and PCR detection. The recovery rate of using virocap filter to concentrate FRNA colipahges was (64 ± 3)%. During the 6-months sampling, 5-160 CFU/100ml of E.coli and 20-580 CFU/100ml of coliforms were constantly detected in GWA; E. coli and coliforms were detected in February and August in GWB, June and August in GWC. The male-specific coliphages ranged in 22-79 PFU/100L (20-60% FRNA) was detected from July to September in GWA; 3 PFU/100L (100% FRNA) in July in GWB; 1-42 PFU/100L (50-100%) from March to September in GWC. However, the RT-PCR detection for enteric viruses was only positive from Jan to March in GWA, GWB and GWC. Hence, the coliphages were not correlated with either coliforms or enteric viruses. Based on this study, FRNA coliphages could be used as an alternate indicator to monitor fecal pollution but does not to indicate the presence or absence of enteric viruses.