P-048. Campylobacter Pathotype Analysis

C. M. Logue, J. S. Sherwood, M. K. Fakhr, E. Lutgen Johnson, S. R. Petermann;
North Dakota State Univ., Fargo, ND.

Annually, Campylobacter is estimated to be responsible for greater than 2.4 million illnesses in the US. C. jejuni and C. coli are typically implicated in greater than 95% of all cases. Although Campylobacter has commonly been associated with human disease and animal hosts, defining a pathotype for strains from various hosts has been limited. In this study C. jejuni and C. coli from turkey (n=45); cattle (n=80); pigs (n=23) and humans (n=17) were evaluated for the presence of 16 genes (cjp02, cjp05, cjp29, cjp32, cjp49, tetO, flaA, flaC, cdtA, cdtB, cdtC, ciaB, cadF, racR, dnaJ and pldA) associated with virulence, resistance and plasmid using polymerase chain reaction. Isolates were also evaluated for the possession of plasmids. Data generated were analyzed based on presence or absence of the genes in each isolate from the different hosts; subjected to cluster analysis and jacquard distances and plotted using a heat map. Analysis found that certain virulence factors were more significantly associated with one strain type than another. The genes ciaB, dnaJ and pldA were not common in C. coli from turkeys, pigs or cattle but were detected in almost 100% of turkey and human C. jejuni strains and approximately 70% or greater of cattle C. jejuni isolates. Plasmid associated genes (cjp02-49), ranged in prevalence from 5 to 100% with the pVir associated gene (cjp29) found more often among turkey, human and cattle C. jejuni strains (range 86-100%) than C. coli strains. The tetO gene was found in 100% of the turkey isolates and 40-75% of other animal isolates. Plasmid prevalence ranged from 48 to 90% among the different host species examined. Heat map analysis of the data showed distinct clustering of isolates based on Campylobacter species and host source. This type of analysis is useful in linking illness with animal host source. Current work is ongoing to expand isolates from different host sources and the selection of genes for pathotyping.