P-035. Comparative Genome Analysis of Vibrio parahaemolyticus Reveals Distinct Genomic Islands between Pandemic and Non-Pandemic Strains

R. G. Gavilan, J. Martinez-Urtaza;
Univ. of Santiago de Compostela, Santiago de Compostela, SPAIN.

Vibrio parahaemolyticus is a marine bacterium that cause gastroenteritis associated with consumption of raw seafood. Currently, this bacterium is considered an emerging foodborn pathogen of which pandemic clones is overly spread out. Although previous studies have compared the strains from the pandemic and non-pandemic groups of V. parahaemolyticus to understand the evolution of the pandemic strain, there is not sufficient information that permit us understand the origin of pathogenic strain. We compared the complete genome sequence of V. parahaemolyticus RIMD 2210633 belonging to pandemic clone serotype O3:K6 and a genomic scaffold of V. parahaemolyticus AQ3810 from a non-pandemic strain serotype O3:k6 isolated in 1983, in order to find genomic islands (GI) and other genetic material acquired by horizontal gene transfer (HGT). Both genome sequences were compared by Mauve v.2.1.1 and MUMmer 3.0 with the aim of obtain differences between pre-pandemic strain contigs and a pandemic strain complete genome. Then this data were ordered using Artemis v.9 and compared with other Vibrio genomes using ACT v.6 to establish the synteny conservation of GI insertional point between vibrios. As part of this comparative analysis based on public information, we found 12 genomic islands, 10 regions in the V. parahaemolyticus RIMD 2210633 (7 in the chromosome I and 2 in the chromosome II) and 2 in chromosome I of the V. parahaemolyticus AQ3810. Four of these genomic islands of V. parahaemolyticus RIMD 2210633 and the two identified in V. parahaemolyticus AQ3810 were novel and unique of these genomes. Genomic islands were mainly related to mobile genes that were phylogenetically related to integrons and transposases from vibrios more than others species. Moreover, we demonstrated certain conservation in the homologous insertion sites associated with GIs. This study indicates the role of HGT in V. parahaemolyticus and how this acquisition may improve its fitness. Likewise, a further study is necessary with the purpose of compare others V. parahaemolyticus genomes that will be sequenced.