N-186. Sediment Archaea Community Depth Profile from Lake Fryxell, McMurdo Dry Valleys, Antarctica
Lake Fryxell (FRX), a perennially ice covered lake in the McMurdo Dry Valleys of Antarctica, has a dynamic methane cycle and has been shown to have
Archaeal communities in the anoxic bottom waters and surface sediments. To
better understand the diversity and contribution of Archaea to biogeochemical
cycles in the sediments, we characterized the Archaeal 16S rRNA gene diversity
in a depth profile of a sediment core collected in November 2006 from the
deepest water depth of FRX. Archaea-specific 16S rRNA gene clone libraries were
constructed; each depth had between 73 to 92 clones (552 clones total). Clones
were grouped by DGGE, RFLP and sequencing into 22 OTUs (>97% similarity).
All depths showed low diversity (5 to 11 total OTUs) with 1 to 3 dominant OTUs.
Two methanogenic archaea (MA) OTUs (designated G3 and G6/7/8), dominate
all depths, except the deepest one (12-14 cm depth) which is dominated by a
Crenarchaeota lineage (designated G11). OTU G3 is most similar to Canditatus Methanosphaerula
paulstris which is associated with the hydrogenotrophic Methanomicrobiales.
OTU G6/7/8 is associated with the acetoclastic Methanosarcinales. The
relative abundance of G3 and G6/7/8 in clone libraries has a reverse
linear relationship (R2=0.79), possibly indicating different
methanogenic substrate distribution in the sediments. Cluster analysis
indicated that the 0-2 cm, 2-4 cm, 4-6 cm, and 8-10 cm depth samples were
highly similar; this observation is further supported by principle components
analysis (PCA) of community composition. Both approaches show the 6-8 cm and
12-14 cm depth samples are disparate communities. The dominance of MA in FRX
sediment archaea community from 0-10 cm depth corroborate previous findings of
high methanogenesis activity in FRX sediments; however the shift to
Crenarchaeota in deeper sediments indicates a distinct change in microbial
driven biogeochemical processes at this depth. Neither the methanogens’
distribution nor the whole Archaea community level similarity have
depth-related trends, possibly suggesting interlayered geochemistry with
different availability of methanogenesis substrates.