N-178. Bacterial Diversity from Seawater Samples at São Paulo Coast Determined by 16S rDNA-PCR

B. C. de Almeida, E. M. Burbano, C. P. Souza, G. G. Martins, I. N. G. Rivera;
Univ. de Sao Paulo, Sao Paulo, BRAZIL.

Before the development of sequence-based methods, it was impossible to know the evolutionary relationships connecting all of life and thereby to draw a universal evolutionary tree. The application of molecular-phylogenetic methods to study natural microbial ecosystems without the traditional requirement for cultivation has resulted in the discovery of many unexpected evolutionary lineages. In the present study we analyzed 451 isolates of viable marine bacteria originating from the seawater samples at the Ubatuba, São Sebastião and Santos, São Paulo Coast with different levels of human activites. These isolates were submitted to Gram staining and 51.7% are Gram-negative bacteria and 48.3% are Gram-positive bacteria. Forty-nine isolates with colorful morphology were submitted to DNA extraction and the template DNA was submitted to subsequent PCR amplification of the 16S rDNA gene. The standardization of the 16S rDNA PCR was performed using conserved primers (fragment to 1533 pb). The PCR products are being purified and sequenced. This study has intended to know the diversity between these isolates and the preliminary results are showing a different species according the human activities in the region.

174/N. Extreme Environments - II

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