K-106. Genomic Response to Triacetonetriperoxide in Saccharomyces cerevisiae W303

V. L. McIntosh, Jr., J. Sanseverino, G. S. Sayler;
Univ. of Tennessee, Knoxville, TN.

S. cerevisiae strain W303 genomic responses to triacetonetriperoxide (TATP) were characterized for the purpose of facilitating toxicogenomic analysis and potential bioreporter construction for detection of this compound. Yeast cultures were exposed to saturated solutions of crystalline TATP for a period of 1 hour followed by total RNA extraction. Three different controls, DMSO, hydrogen peroxide and a mixture of hydrogen peroxide and acetone were used to assess the response such that three pairwise comparisons were generated (DMSO vs TATP, Peroxide vs TATP, and Mixture vs TATP). Messenger RNA was hybridized to a single color Affymetrix Yeast 2.0 gene chip array for analysis. The response was analyzed based on fold changes in mRNA abundance from each of the pairwise comparisons. There were 211 and 230 genes with significant changes in expression when compared to DMSO and Mixture controls respectively, Of these, 185 genes were common to both treatments. However, the peroxide control resulted in only 11 significant genes. There were 5 genes common to all three comparisons. A significant portion of the genes observed were of unknown function. The responses observed primarily consisted of decreased expression of genes associated with cellular metabolism, protein synthesis, and metabolism of sulfur compounds. Significant increase in genes associated with osmotic stress, membrane synthesis and repair, and detoxification were observed in all three comparisons. The DMSO control in particular illuminated an expression pattern consistent with oxidative stress.

173/K. Functional Genomics

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