C-176. Do Blood Cultures from Children Require a Pediatric Formulation?

M. E. Reller1, S. Mirrett2, L. B. Reller2;
1Johns Hopkins Med. Inst., Baltimore, MD, 2Duke Univ. Med. Ctr., Durham, NC.

Objectives: BACTEC PEDS PLUS/F (Becton Dickinson, Sparks, MD), designated Peds Plus (PP), is an aerobic blood culture medium with resins designed to detect bacteremia in pediatric patients. When pediatric bottles are used for adult patients, inadequate volumes of blood are cultured. A dual purpose bottle could simplify inventory and promote age and weight appropriate volumes of blood for culture for both children and adults. Therefore, to determine whether a pediatric medium is necessary, we compared the performance of Peds Plus with that of an aerobic medium with resins formulated for adults (PLUS Aerobic/F), designated Adult Plus (AP), for recovery of microorganisms from blood and their time to detection. Methods: All bottles were weighed before inoculation and upon receipt in the laboratory; only bottles filled with comparable volumes (within 50% if <5 mL and within 20% if >=5 mL was collected)) of blood were compared. Results: Of 12,172 complete sets received, 9,419 had comparable volumes of blood. Of 585 clinically significant (based on previously published criteria) isolates, 428 were detected in both bottles, 80 in PP only, and 77 in AP only (P=NS). No microorganism was detected significantly more frequently in one medium versus the other. Of 428 cultures positive in both bottles, the mean time to detection of pathogens was 19.5 hours in PP and 20.1 hours in AP. False-positive instrument signals were detected in 24 of 12,896 (0.2%) PP bottles received and 16 of 12,491 (0.1%) AP bottles. Conclusions: We conclude that PEDS PLUS/F and PLUS Aerobic/F bottles are comparable for recovery of microorganisms from pediatric patients and that PLUS Aerobic /F bottles could be used for blood cultures in the BACTEC system for both children and adults.