C-150. Intra and Inter Laboratory Comparison of the Becton Dickinson ProbeTec and VIPER™ Systems for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae

R. L. Sautter1, D. A. Bankert2, R. L. Drechsel1, B. Goad1, A. E. Crist, Jr2;
1Carolinas Lab. Network, Mecklenburg County Hlth. Dept., Charlotte, NC, 2York Hosp., York, PA.

Background: The objective of this study is to determine the intra- and inter-laboratory reliability of Becton Dickinson ProbeTec System and VIPER™ instrument for testing samples for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC). Inter- laboratory comparisons were performed at Carolinas Laboratory Network (CLN) between the ProbeTec and Viper system as well as comparing both systems to ProbeTec results from York Hospital (YH). Methods: Both swab and urine samples were collected according to the manufacturer’s recommendations then tested and frozen for further evaluations. Assays were performed according to manufacturer’s instructions. A total of 685 comparisons were completed. Results: Complete agreement was achieved between CT and GC on both the ProbeTec and Viper tested at CLN. Using the ProbeTec instrument only two discrepancies were noted during the intra-laboratory comparison for CT. Two samples were positive at CLN for CT and negative at YH. No intra-laboratory discrepancies were noted for GC using the ProbeTec instrument. Four inter- laboratory discrepancies were found between the ProbeTec and Viper systems. Two were for CT and two for GC. The ProbeTec system compared between sites with a sensitivity of 100% and a specificity of 95% and 100% and 100% respectively for the detection of CT and GC. Overall the ProbeTec compared to the Viper system with a sensitivity and specificity of 100% and 98% for CT and 98% and 99% for GC. Conclusions: In conclusion, there was > 98% agreement between the two systems in detecting CT and GC. It is our opinion that both the Becton Dickinson ProbeTec and VIPER™ systems show a high degree of correlation with each other. Both intra and inter laboratory comparisons were excellent.