B-200. Complete Genome Sequence of Finegoldia magna, an Opportunistic Pathogen in Gram-Positive Anaerobic Cocci

T. Goto1, A. Yamashita2, H. Hirakawa3, M. Matsutani3, K. Ohshima2,4, H. Toh2,5, K. Todo1, K. Miyamoto1, S. Kuhara3, M. Hattori2,4,5, T. Shimizu6, S. Akimoto1;
1Wakayama Med. Univ., Wakayama, JAPAN, 2Kitasato Univ., Kanagawa, JAPAN, 3Kyushu Univ., Fukuoka, JAPAN, 4Univ. of Tokyo, Chiba, JAPAN, 5RIKEN Genomic Sci. Ctr., Kanagawa, JAPAN, 6Kanazawa Univ., Ishikawa, JAPAN.

Background: Finegoldia magna (formerly Peptostreptococcus magnus) is a member of the Gram-positive anaerobic cocci (GPAC), a major part of the normal human flora. F. magna is also recognized as an opportunistic pathogen responsible for various infectious diseases such as diabetic foot infections. Here, we report the complete genome sequence of F. magna ATCC 29328, originally isolated from an abdominal wound. Methods: The nucleotide sequence was determined by a whole-genome shotgun strategy. Protein coding regions were identified using GenomeGambler 1.51, CRITICA, GeneHacker, and Glimmer 2.0 programs. The characteristic motifs in all protein-coding sequence were detected by the Pfam, COGs, PSORT, and SignalP analyses. Sortase homologs and their substrates from the ATCC 29328 genome were identified in silico according to methods described by Comfort D et. al. (Infect. Immun., 72, 2710-2722, 2004). Results and Discussion: The ATCC 29328 genome consists of a 1,797,577-bp circular chromosome and an 189,163-bp plasmid, possessing 1,631 and 182 protein-coding genes, respectively (See accession nos. AP008971 and AP008972 in the GenBank database). The metabolic maps constructed based on the genome information confirmed that most F. magna strains cannot ferment most sugars, except fructose. The chromosome encodes three homologs of albumin-binding protein, a known virulence factor useful for antiphagocytosis. Interestingly, the plasmid also encodes one albumin-binding protein homolog with two albumin-binding modules. A unique feature of the genome is that F. magna encodes many sortase genes, of which substrates may be involved in bacterial pathogenesis, such as antiphagocytosis and adherence to the host cell. The plasmid encodes seven sortase and seven substrate genes, whereas the chromosome encodes four sortase and 19 substrate genes. These plasmid-encoded sortases may play important roles in the pathogenesis of F. magna by enriching the variety of cell wall anchored surface proteins. This is the first complete genome sequence among GPAC, and we believe our data will be of great use for genetic studies of other GPAC as well as F. magna.